Manuel Alves-Ferreira |
Brief CV
Miguel Alves-Ferreira is a Junior Research at Instituto de Investigação e Inovação em Saúde (i3S) and an Invited Auxiliary Professor at ICBAS- Univ. Porto. His main research interest is in the genetic of complex and mendelian genetic diseases, focusing nowadays in Primary Headaches and Familial Amyloidotic Polyneuropathy (FAP), using a multi-omics approach. MAF completed PhD in Biomedical Sciences (2019) at ICBAS/IBMC-i3S University of Porto in collaboration with the The Scripps Research Institute (TSRI, California). Postgraduate (2017) in Graduate Research for Advance Study Program at TSRI, USA. Currently a co-PI in project Interreg V A España-Portugal (POCTEP). |
TOOTH AGENESIS, TEMPOROMANDIBULAR DISORDERS AND MIGRAINE: A GENETIC.
Orofacial structures development are a complex mechanism regulated by sequential and reciprocal epithelial-mesenchymal interactions, controlled by activators and inhibitors involved in several pathways. Disturbances in these signaling cascades can lead to abnormalities from odontogenesis to the musculoskeletal system, resulting in alterations in the formation of the normal teeth number and in pain. Despite environmental disturbances, many multifactorial oral diseases (including tooth agenesis and temporomandibular disorders (TMD)) are mainly genetically controlled. To date, more than 200 genes have been identified in tooth development. Primary headache disorders (PHD), specifically migraine, are known to be strongly associated to TMD, sharing some patterns of orofacial pain. Besides that, both disorders have a major genetic component associated to the onset of the pathophysiological mechanisms, which are already studied. Mutations/variants in any of these strictly balanced signaling cascades may cause arrested odontogenesis and/or other oral defects. Thus, our studies intend to study the genetic alterations that can trigger oral diseases development and their comorbidities. The importance of these studies resides in the opportunity to explore recent advances in the genetic profile of these pathologies that may serve as a therapeutic targets and in future diagnostic and/or monitoring techniques that could aid the treatment in the early stages. |
Fernando Guerra |
Brief CV·
Full Professor from the Faculty of Medicine of the University of Coimbra Director of the Center of Innovation and Research in Oral Sciences Director of the Institute of Implant Dentistry and Prosthodontics |
Clinical Studies in Implant Dentistry – Relevant aspects to consider
This presentation has the purpose to address important aspects in the design and publication of clinical studies in implant dentistry, such as: organizationals issues, primary and secondary outcomes, flowcharts, size calculation, methodologies of analysis, explanation of results, etc. Randomized clinical trails are emphasized with examples of studies performed at the Center for Innovation and Research in Oral Sciences from the Faculty of Medicine the University of Coimbra. |
Arthur R. G. Cortes |
Brief CV
Prof. Arthur Rodriguez Gonzalez Cortes, DDS, MSc, PhD is an Associate Professor of Oral Radiology and Digital Dentistry at the Department of Dental Surgery, Faculty of Dental Surgery, University of Malta, where he conducts research focused mostly on Digital Dentistry. He got his DDS, MSc and PhD degrees from University of São Paulo (Brazil), and completed a one-year postdoctoral fellowship in Radiology at Harvard University, (Boston, USA). He has published more than 50 international scientific articles in journals indexed on the pubmed website, most of them from funded projects. In addition, Prof. Cortes is an oral implantologist and radiologist with 15 years of experience. As an ITI director, Prof. Cortes has been lecturing on Digital Dentistry as an invited speaker in different countries such as Brazil, Spain, Denmark, Korea, Peru and Malta, including a visiting professor appointment at University of Okayama (Japan). |
Aspects of CAD-CAM systems influencing digital workflow outcomes
The continuous development of computer-aided design and computer-aided manufacturing (CAD-CAM) has led to the creation of several new techniques and methodologies in oral rehabilitation. In consequence, recent studies have aimed at addressing the accuracy of CAD-CAM methods and its impact on the quality of the resulting CAD-CAM prostheses. This lecture will offer detailed information on CAD-CAM accuracy, which can also be affected by factors associated with the image-acquisition procedures. |
Rita Pereira |
Brief CV |
Comparison between flowable and conventional resin-matrix composites regarding chemical composition and polymerization.Introduction:
Technological development of resin-matrix composites has provided several options of commercial materials for enhanced esthetical outcomes. Low viscosity (flowable) resin-matrix composites are mostly indicated for preventive restorations, such as: minimally invasive occlusal class I or II restorations, pit and fissure sealants and inner layer for class II posterior resin-matrix composite placement in sealing of gingival margins. However, the low filled flowable resin-matrix composites are more susceptible to wear in stress bearing areas and therefore may not be the clinicians’ first choice. The development of new organic matrix and inorganic fillers might overcome current limitations of flowable resin-matrix composites. Aim: The aim of this study was to evaluate the shape, chemical composition and size of inorganic fillers in the microstructure of recent conventional and flowable resin-matrix composites by using optical and scanning electron microscopy. Materials and Methods: Six resin-matrix composites with different inorganic content were prepared. The flowable resin-matrix composites showed the following filler content in weight (%wt): 76, 62.5, and 60%. Two traditional nanohybrid resin-matrix composites specimens with 80 and 89wt% filler content in weight and one submicron-scale hybrid resin-matrix composite with 74 wt% fillers were also assessed. Disc-shaped specimens with 2mm thickness and a diameter of 6mm were light cured for 40s according to the standard guidelines. Specimens were cross-sectioned using graded SiC grit-papers under automatic polishing and distilled water lubrication. After 10 minutes of ultrasonic cleaning in propanol and distilled water, surfaces were inspected by optical microscopy followed by scanning electron microscopy (FEGSEM, FEI Nova 200) on secondary (SE) and backscattered electron (BSE) modes coupled to energy dispersive spectroscopy 5-15 kV within magnification up to 50,000 to accurate measurements of nanoparticles size. Fillers were identified by using EDAX-Pegasus X4M (EDS/EBSD) coupled to the FEGSEM unit. Regarding polymerization was also investigated the effect of inappropriate polymerization on oral tissues and its effects of oral and systemic toxicity. Results: The flowable resin-matrix composites with 62.5 wt% fillers showed irregular particles with particle size ranging from 0.1 up to 3.0 µm. Another flowable resin-matrix composite with 60 wt% fillers showed amorphous silica and zinc oxide particles ranging from 0.02 up to 1.4 µm. The third flowable resin-matrix composite revealed nanoparticles with average particle size of 20-40nm and glass ceramic irregular particles with 1 μm. Nano-hybrid resin composites revealed silica nano-particles at 20-40 nm and micro-scale particles (zirconia glass fillers, barium glass, prepolymerized fillers and ytterbium fluoride) ranging from 0.1 to 3.0 μm. Submicron-hybrid resin-matrix composite showed particles range between 0.02 and 1.5 μm. Defects, such as micro-scale voids and pores, were also found revealing the handling sensitivity of the technique. Regarding polymerization, it was also noted that mechanical properties and biocompatibility are correlated to DC, which depends on sufficient amount of light reaching all restoration, it was also verified that increase time polymerization exposure could raise in pulpal temperature and polymerization devices could cause cellular lesions. The degree of conversion of resin matrix composites affects the release of potentially toxic substances that are present within the resin composite. Conclusion: The flowable resin-matrix composites with 76 wt% with nanosized fillers revealed a similar type, shape and high percentage of fillers when compared with high filled traditional nanohybrid composites. Regarding filler´s constitution, highly filled flowable composites were morphologically similar to the traditional resin-matrix composites. When compared with submicron-hybrid resin matrix composites, highly filled flowable resin-matrix composites revealed organized nanosized particles with irregular shapes which allow a highly loaded resin composite. Regarding polymerization, it could be conclude that doubling the exposure time may significant increase pulpal temperature, which should be considered by the clinicians when they want to achieve higher DC% for better clinical performance of the restoration. The choice of polymerization device is extremely important to achieve a reduction of composite toxicity is possible if the curing mode is adapted to the used composite. |
Valter Fernandes |
Brief CV· www.linkedin.com/in/vfendodontics· 0000-0003-4432-5424
· Invited Professor Post-Graduation Program on Endodontics at IUCS – CESPU · Monitor of the Conservative Dentistry Department at IUCS – CESPU · Student of the Doctoral Program in Biomedical Sciences – IUCS – CESPU –2020/2024 · Post-Graduation on Endodontics of Dental School of University of Lisbon – 2015 · Post-Graduation on Endodontics of IUCS CESPU – 2010 · Graduation in Dentistry of Dental School of University of Porto – 2008 · Member Direction board of the Portuguese Society of Endodontics – 2020/2023 · Author scientific publications on Endodontics, Conservative Dentistry and Dental Materials · Clinical Practice dedicated to Microscopic Endodontics since 2010. |
Resin cement around commercial glass fiber-reinforced composites posts.
Anatomical differences along the root canal cause an variable and unpredictable distance between the post and the intracanal dentin surface. Such space is filled with different volume of resin-matrix cement after cementation. Thick layers of resin cements are prone to defects like macro- and microscale pores, cracks, and voids that create stress concentration zones leading do root fractures. The main objective of our study was to evaluate the microstructure and spacial distribution of fibers of glass fiber-reinforced composites (GFRC) posts to tooth root intracanal dentin by microscopic analysis. Extracted human premolars with completely formed apex were used in this study. The anatomic crowns were sectioned, and all teeth were endodontically treated. Groups of specimens were divided according to the cementation with different endodontic post systems as follow: A) ParaPost Fiber LuxTM (Coltène, Whaledent Inc, USA); B) Rebilda GTTM (VOCO, Germany); C) Angelus ExactoTM (Angelus, Brazil). After cementation, specimens were cross-sectioned at 90 degrees relative to the plane of the GFRC post to resin-matrix cement interface. Then, specimens were inspected by optical microscopy (OM) (Leica DM 2500 MTM, Leica Microsystems, Germany) and scanning electron microscopy (SEM) (JSM-6010 LV, JEOL, Japan). Microscopic analyses revealed a wide variation of resin-matrix cement thickness around the endodontic GFRC posts. Also, the shape of the root canal preparation caused a variation in the thickness of the remnant tooth tissues. Defects such as micro-scale pores, cracks, and voids were also detected by OM and SEM analyses. The root canal preparation can promote a decrease in the thickness of the remnant tooth tissues that can increase the risks of clinical failures by fracture. Keywords: Fiber posts; Rehabilitation; Dentin Thickness; Fitting |
8th April 2022Conference on Advances in Research on Oral Cancerconvened by UNIPRO and the WHO Collaborating Centre on Oral Cancer
Alfândega do Porto – Sala D. Luís |
Adalberto Miranda-Filho |
Brief CV
Dr Adalberto Miranda Filho is as an applied epidemiologist with a track record in evaluating the impact of cancer and its causes across populations internationally. As background, he holds an MPH and a PhD in Epidemiology in the Oswaldo Cruz Foundation (FIOCRUZ-RJ). Over the last years, he has been working at IARC, as a Scientist (Epidemiologist) within the IARC Monographs Programme (IARC/IMO), responsible for identifying environmental factors that are carcinogenic hazards to humans. Prior to the above post, he held an IARC Postdoctoral Fellowship within the Cancer Surveillance Branch (IARC/CSU). |
The incidence and trends of oral and oropharyngeal cancer in Europe.
We presented here the European statistics on oral cavity and oropharynx cancers using the comprehensive set of national estimates, as well as the high-quality recorded data provided by population-based cancer registries (PBCR) in Europe. Overall, cancer in the oral cavity and oropharynx represent the 16th and 24th most common malignant neoplasm in Europe, respectively, in 2020. Estimated age-standardized incidence rates for oral cavity cancer was 13.3 and 1.1 for oropharynx. Trends are diverse, with oral cavity and pharynx (combined) cancer incidence rates continuing to decrease for males, and incidence rates still stable in females, e.g., Italy, France, Germany, Spain, and U.K. Other countries, namely Portugal and Poland, ncidence rates have either slightly increased or are still stable for males and females, since the 2000s. Global and regional descriptions of international patterns and trends in oral and oropharyngeal cancer are informative in providing insight into the shifting epidemiologic patterns and the potential prevention of these tumours. |
Pablo Ignacio Varela Centelles |
Brief CV
Lecturer in Oral Surgery at the Medical & Dental School of the University of Santiago de Compostela (Spain) and head of primary dental care units of the Galician Health Service for 25 years, he qualified as a dental surgeon at the Complutense University in Madrid. Later, he obtained a master’s degree from the University of Sheffield (UK) in Periodontology. He also holds a master’s degree in Public Health & Community Dentistry from the University of Santiago de Compostela, where he was granted a PhD with a dissertation on survival to oral cancer and its associated factors. He has authored 100+ scientific papers, supervised 12 PhD thesis, and leaded 5 competitively funded research projects and several contracts with the private sector. He was awarded 5 patents. Dr. Varela-Centelles is the current leader of the Dermatology and Craniofacial Pathology research group of the Santiago de Compostela Health Research Institute. |
Delays in diagnosis of oral cancer in primary care- a systematic review.
Oral cancer incidence does not seem to be under control and a high proportion of these patients keep being diagnosed at advanced disease stages. Several factors have been linked to this phenomenon, including diagnostic delay. The most important component of oral cancer diagnostic delay is the time needed for the patient to contact a healthcare professional once the person has noticed a bodily change. The aim of this systematic review was to analyse what happens since this first contact until referral for hospital care. To achieve this goal, we checked four databases (PubMed®, Embase®, Scopus®, and SciELo®) to obtain 727 references. Finally, 11 papers (mostly Europeans and published in this century) reporting on 1,778 patients were selected. The median length of the primary care interval ranged from 6 to 39.5 days. These patients were mainly seen by family physicians who needed from one to six consultations to refer the case for specialised care. The odds ratio for being referred at an early stage ranged from 0.10 (95% CI: 0.01 – 0.99) to 1.36 (95%CI: 0.52 – 3.53). |
António Mano Azul |
Brief CV
Antonio Mano Azul, MD, DDS, MsC, Specialist in Stomatology Professor and co- Head of Department of Oral Surgery, Oral Medicine and Maxillofacial Surgery. Instituto Universitario Egas Moniz, Monte da Caparica, Portugal (Egas Moniz – Cooperativa de Ensino Superior) Coordinator of the National Program for Promotion of Oral Health and PIPCO (Intervention Program for Oral Cancer), General Directorate of Health (DGS), Ministery of Health. Portugal Chief Dental Officer Founder member and Past-President of the European Association of Oral Medicine. President of the Portuguese Academy of Oral Medicine. |
Recent public health interventions in Portugal to control oral cancer: Screening & Early detection.
Portugal started a couple of years ago a public program to improve the rate of early detection of oropharyngeal cancer and OPML and consequently improve the 5 years survival rate and the patients quality of life after treating oral cancer. A “collateral side-effect” was to improve literacy on oropharyngeal cancer of Portuguese dentists and general medical practitioners, involving them as main players of this National Program (PIPCO). We are now in a phase of improving the PIPCO software after testing it for 5 years now and of launching in the near future a literacy effort for the population with the help of the EU Mobile App for Cancer Prevention (Europer’s Beating Cancer Plan). |
José M. Aguirre |
Brief CV
ACADEMIC FORMATION |
PROGNOSTIC MARKERS OF ORAL CANCER: A PATHOLOGIST’S VIEW.
Oral cancer is an important malignancy in humans and squamous cell carcinoma (SCC) is the most frequent type, accounting for over 90%. OSCC still has a very poor prognosis with survival at 5 years of less than 50%. In this important oral disease, the histopathological study is crucial for its early diagnosis and to establish a prognosis. |
Hassan Bousbaa |
Brief CV
Hassan Bousbaa is an Associate Professor of Cellular and Molecular Biology at the University Institute of Health Sciences (IUCS-CESPU). PhD in Life and Health Sciences (University of Paris-Est Créteil, France). His post-doc research focused on molecular genetics of mitosis, at the University of Porto, to unveil the mechanisms of cell division, and understand how errors in chromosome segregation arise and lead to genomic instability in cancer. He was part of the pioneering researchers that identified and characterized the genes involved in the mitotic checkpoint signaling, with great impact in the field. His current interests at UNIPRO include exploiting the clinical relevance of this information to identify novel cancer diagnostic and prognostic markers, as well as to develop novel therapeutic strategies to kill cancer cells or to increase their sensitivity to antimitotic agents. Hassan published >60 peer review publications (Scopus h-index: 21). |
The spindle assembly checkpoint in oral cancer.
Abnormal chromosome number, or aneuploidy, is a common feature of human solid tumors, including oral cancer. Deregulated spindle assembly checkpoint (SAC) is thought as one of the mechanisms that drive aneuploidy. In normal cells, SAC prevents anaphase onset until all chromosomes are correctly aligned at the metaphase plate, thereby ensuring genomic stability. Significantly, the activity of this checkpoint is compromised in many cancers. While mutations are rather rare, many tumors show altered expression levels of SAC components. Genomic alterations such as aneuploidy indicate a high risk of oral cancer and cancer-related mortality, and the molecular basis of these alterations is largely unknown. Here, I will address the current understanding of the SAC molecular mechanisms, and discuss how this signaling circuitry can be exploited as drug targets to develop effective cancer therapies. The potential of SAC targeting as promising strategy against oral cancer will be highlighted. |